Irs4 Antibody | 43-180

Irs4 Antibody | 43-180 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat

Immunogen: The immunogen for this antibody is: C-DRGTKERKEAKEVRD

Research Area: Immunology

Tested Application: E, WB, IF

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Heart lysates (calculated MW of 130kDa according to NP_034702.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144 (4) :1211-8. PMID: 12639902) . Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at ~39kDa. This band was successfully blocked by incubation with the immunizing peptide. This product has been successfully used in WB on Mouse: Ikink GJ et al, Nat Commun. 2016 Nov 23;7:13567. PMID: 27876799.Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25;14:831. PMID: 24274089.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Approx 150kDa

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: Irs4, insulin receptor substrate 4, IRS-4, RP23-415G1.3, phosphoprotein of 160 kDa, pp160

User Note: N/A

BACKGROUND: N/A