Isopeptidase T (short form), human recombinant | 4861

Ubiquitin C-terminal hydrolases (UCHs) are a family of cysteine hydrolases that catalyze the UBPs hydrolyze isopeptide bonds between ubiquitin and the substrate protein or within polyubiquitin chains. Human isopeptidase T (isoT) is an UBP capable of binding ubiquitin that specifically disassembles free polyubiquitin chains linked through isopeptide bonds involving the K48 of ubiquitin into ubiquitin monomers.

4861 | Isopeptidase T short form human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: USP5; Ubiquitin-specific protease 5

Alternates names: USP5; Ubiquitin-specific protease 5

Taglines: A ubiquitin-specific processing protease

NCBI Gene ID #: 20296

NCBI Gene Symbol: MCP-1

Gene Source: Human

Accession #: P10148

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by measuring the dose dependent chemotaxis with human THP-1 cells. The ED is in the range of 1-10 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 93.4 kDa

Concentration: 2.0 mg/ml

Appearance: Liquid

Physical form description: Supplied at a concentration of 2.0 mg/ml in 50 mM Tris, 10 mM -mercaptoethanol, pH 7.5.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A