JAK2 Antibody | 8085

JAK2 Antibody | 8085 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Pig: (100%) , Chicken: (82%)

Immunogen: JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2.
The immunogen is located within the last 50 amino acids of JAK2.

Research Area: Stem Cell, Apoptosis

Tested Application: E, WB, IHC-P

Application: JAK2 antibody can be used for detection of JAK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.

Positive Control 1: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 125 kDa
Observed: 125 kDa

Validation: N/A

Isoform: N/A

Purification: JAK2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: JAK2 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: JAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Janus kinase 2, JTK10, THCYT3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1, 2) . JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3) . Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4) .