JE (MCP-1), murine recombinant | 4207

Murine JE (also known as Macrophage/Monocyte Chemotactic Protein) is a 13.8 kDa protein containing 125 amino acid residues. It plays an important role in the inflammatory response of blood monocytes and tissue microphages.

4207 | JE MCP-1 murine recombinant DataSheet

Biomolecule/Target: MCP-1/CCL2

Synonyms: Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine (C-C motif) ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11, Immediate-early serum-responsive JE protein.

Alternates names: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.

Taglines: Chemoattractant for monocytes and basophils

NCBI Gene ID #: 1435

NCBI Gene Symbol: CSF1

Gene Source: Human

Accession #: P09603

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: The EC50 is typically 0.5 to 4 ng/mL for this protein with specific activity determined by the dose dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line)

Results: 0.5 to 4 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein has 223 amino acids (Glu 33 - Arg 255) with a 6x-His tag at C-terminus, and a calculated MW of 26 kDa. The protein migrates to 35-45 kDa under reducing and non-reducing conditions (SDS-PAGE) due to glycosylation

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Amino acid sequence: N/A