JMJD3 Antibody | 6657

JMJD3 Antibody | 6657 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-JMJD3 antibody (6657) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human JMJD3.
The immunogen is located within amino acids 1370-1420 of JMJD3.

Research Area: Stem Cell

Tested Application: E, WB, ICC, IF

Application: WB: 0.25-4 μg/mL; ICC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: This antibody is specific for JMJD3 and will not recognize other JMJD proteins.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide

Positive Control 3: Cat. No. 1204 - K562 Cell Lysate

Positive Control 4: Cat. No. 1288 - A-20 Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 180
Observed: 180 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar JMJD3 expression profile in human, mouse and rat cell lines detected by two independent anti-IFN-beta antibodies that recognize different epitopes, 6657 against C-terminus domain and 14-618 against N-terminus domain.  IFN-beta proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human JMJD3 has 2 isoforms, including isoform 1 (1682aa, 181kD) and isoform 2 (1643aa, 177kD) . Mouse JMJD3 has one isoform (1641aa, 176kD) and rat JMJD3 also has one isoform (1356aa, 146kD) . 6657 can detect human, mouse and rat.

Purification: JMJD3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: JMJD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: JMJD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: JMJD3 Antibody: JMJD3, JMJD3, KIAA0346, Lysine-specific demethylase 6B, JmjC domain-containing protein 3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: JMJD3 Antibody: The Jumonji domain-containing protein 3 (JMJD3) functions as a trimethylation-specific demethylase, converting the trimethylated histone H3 Lys27 residue to the dimethylated form, and is thought to also function as a transcriptional repressor. JMJD3 plays a central role in regulation of posterior development, by regulating HOX gene expression. It is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. JMJD3 can also interact with and demethylate p53, resulting in its stabilization and localization to the nucleus in mouse embryo fibroblasts during neural stem cell differentiation.