JNK1, Active | 7757

JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity.

7757 | JNK1 Active DataSheet

Biomolecule/Target: N/A

Synonyms: JNK, mitogen-activated protein kinase 8

Alternates names: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.

Taglines: Kinases that bind and phosphorylate c-Jun

NCBI Gene ID #: 1435

NCBI Gene Symbol: CSF1

Gene Source: Human

Accession #: P09603

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The EC50 is typically 0.5 to 4 ng/mL for this protein with specific activity determined by the dose dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line)

Results: 0.5 to 4 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein has 223 amino acids (Glu 33 - Arg 255) with a 6x-His tag at C-terminus, and a calculated MW of 26 kDa. The protein migrates to 35-45 kDa under reducing and non-reducing conditions (SDS-PAGE) due to glycosylation

Concentration: 0.1mg/ml

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Amino acid sequence: N/A