KLRA1 Antibody | 4999

KLRA1 Antibody | 4999 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: KLRA1 antibody was raised against a 13 amino acid synthetic peptide near the center of human KLRA1.
The immunogen is located within amino acids 140 - 190 of KLRA1.

Research Area: Immunology

Tested Application: E, WB, ICC, IF

Application: KLRA1 antibody can be used for detection of KLRA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.

Positive Control 1: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 2: Cat. No. 17-010 - HEK293 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: KLRA1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: KLRA1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: KLRA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: KLRA1 Antibody: Ly49, KLRA#, KLRA1, LY49L, Ly-49L

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: KLRA1 Antibody: KLRA1 (also known as Ly49L) is a member of the LY49 family of receptors in Natural Killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors) , or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylation of several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells.