KLRG1 Antibody [2F1] | 98-965

KLRG1 Antibody [2F1] | 98-965 | Gentaur UK, US & Europe Distribution

Host: Hamster

Reactivity: Mouse, Human

Homology: N/A

Immunogen: IL-2-activated NK cells (A-LAK cells) from B6 (H-2b) mice

Research Area: Innate Immunity, Immunology

Tested Application: IHC-Fr, Flow

Application: KLRG1 Antibody [2F1] for use in flow cytometry, western blotting, and immunoprecipitation assays.

Specificiy: KLRG1

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [2F1]

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: MAFA, killer cell lectin-like receptor G1, mast cell function-associated-antigen

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The monoclonal antibody 2F1 reacts with mouse killer cell lectin-like receptor G1 (KLRG1; formerly known as mouse MAFA) , a homodimeric member of the lectin-like type 2 transmembrane receptor family that contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. The 2F1 antibody stains 30-60% of NK1.1+CD3- splenocytes, and a small fraction of T cells in all mouse strains tested (C57BL/6, BALB/c, 129/J, C3H.SW, AKR/J, SJL) . Cell surface expression of KLRG1 is up-regulated by expression of MHC class I molecules. The effect of MHC class I expression is indirect, and can be mediated by interactions with class I-specific Ly49 inhibitory receptors.