KSR Antibody (N-term E174) [APR17138G]

KSR Antibody (N-term E174) [APR17138G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This KSR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-194 amino acids from the N-terminal region of human KSR.

Cellular Localisation: Cytoplasm. Membrane; Peripheral membrane protein. Cell membrane {ECO:0000250|UniProtKB:Q61097}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q61097}. Cell projection, ruffle membrane {ECO:0000250|UniProtKB:Q61097}. Endoplasmic reticulum membrane. Note=In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery.

Molecular Weight: 102160

Clone: Polyclonal

Gene Name: KSR1

Gene ID: 8844

Function: Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases (By similarity) . Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF; upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2 (PubMed:29433126) . Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP (By similarity) . Its kinase activity is unsure (By similarity) . Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown (By similarity) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Kinase suppressor of Ras 1, KSR1, KSR