LC3 (phospho-Thr50) polyclonal Antibody | AP4013

LC3 (phospho-Thr50) polyclonal Antibody | AP4013 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB

Application Range: WB: 1:500~1:1000

Background: Two forms of LC3β, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: LC3 (phospho-Thr50) polyclonal Antibody detects endogenous levels of LC3A and LC3B protein only when phosphorylated at Thr50. This Antibody also recognizes LC3C when phosphorylated at the corresponding residues.

Molecular Weight: ~ 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II; MAP1LC3A

Immunogen: Synthetic phosphopeptide derived from human LC3 around the phosphorylation site of Threonine 50.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: