Legumain/Asparaginyl Endopeptidase | E21-371

Legumain/Asparaginyl Endopeptidase | E21-371 | Gentaur UK, US & Europe Distribution

Short Description: Recombinant Human Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Ile18-Tyr433 is expressed with a 6His tag at the C-terminus.

Shipping: Dry ice/ice packs

Formulation: Supplied as a 0.2 m filtered solution of 20mM TrisHCl, 150mM NaCl, 10% Glycerol, pH 8.0.

Storage: Store at < -20C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Reconstitution: N/A

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/g (1 IEU/g) as determined by LAL test.

Background: Legumain is a lysosomal cysteine protease which is a member of the peptidase C13 family. Though it is found in many tissues, it is highly expressed in the kidney, heart, and placenta. Legumain has a strict specificity for hydrolysis of asparaginyl bonds and can also cleave aspartyl bonds slowly, especially under acidic conditions. Over-expression Legumain in tumors is significant for invasion and metastasis. In addition, Legumain may be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system and negative regulation of neuron apoptosis.

Species: Human

Expression System: Human cells