LEMD3 Antibody | 6603

LEMD3 Antibody | 6603 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: LEMD3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human LEMD3.
The immunogen is located within the last 50 amino acids of LEMD3.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: LEMD3 antibody can be used for detection of LEMD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of LEMD3 are known to exist; this antibody will detect all isoforms. LEMD3 antibody is predicted to not cross-react with LEMD1 and LEMD2

Positive Control 1: Cat. No. 1320 - Human Colon Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: LEMD3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: LEMD3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: LEMD3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: LEMD3 Antibody: MAN1, MAN1, Inner nuclear membrane protein Man1, LEM domain-containing protein 3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: LEMD3 Antibody: LEMD3 (LEM domain-containing 3) is a lem-domain containing protein, also known as MAN1, whose loss of function results in disorders characterized by osteopoikilosis, Buschke-Ollendorff syndrome, and melorheostosis. LEMD3 is an inner nuclear membrane protein that interacts with BMP and activin-TGF-beta receptor-activated Smads and antagonizes TGF-beta signaling in human cells.