Leptin, rat recombinant | 4368

Leptin (Anti-Obesity Protein) is the protein product of the ob (obese) gene in mice and seems to be involved in appetite control. The ob gene is expressed in adipose tissue and is thought to regulate the body’s fat stores. Mice with the ob/ob genotype also develop a form of diabetes similar to type II (non-insulin dependent) diabetes. Rat Leptin is a 16.2 kDa protein containing 147 amino acid residues.

4368 | Leptin rat recombinant DataSheet

Biomolecule/Target: Leptin

Synonyms: OB Protein, Obesity Protein, OBS, Obesity factor.

Alternates names: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.

Taglines: Adipose-derived hormone which regulates energy intake & energy expenditure

NCBI Gene ID #: 3553

NCBI Gene Symbol: IL1B

Gene Source: Human

Accession #: P01584

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.02 to 0.08 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.

Results: 0.02 to 0.08 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18 and 25 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from 4.5 µM NaHCO.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A