Description
Leptin Receptor Antibody | 45-084 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Mouse, Rat
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-TQDDIEKHQSDAG
Research Area: Obesity
Tested Application: E, WB, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 132kDa according to Human NP_002294.2 and 130.8kDa according to Mouse NP_666258.2 and Rat NP_036728.1) . The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration:0.1-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Heart. Recommended concentration: 4ug/ml.
Specificiy: This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1) .
Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: LEPR, leptin receptor, CD295, OBR, OB receptor, LeprB
User Note: N/A
BACKGROUND: N/A