LIF, human recombinant | 4923

Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.

4923 | LIF human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Leukemia Inhibitory Factor

Alternates names: T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.

Taglines: A cytokine that affects cell growth by inhibiting differentiation

NCBI Gene ID #: 3558

NCBI Gene Symbol: IL2

Gene Source: Human

Accession #: P60568

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: EDis typically 0.2 to 2 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).

Results: 0.2 to 2 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A