LIGHT, Human Recombinant | P1291

LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD+8 tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to active NFB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells. Recombinant human LIGHT has a calculated mass of 19.3 kDa containing 177 amino acid residues. Due to glycosylation LIGHT migrates between 20.0-22.5 kDa by SDS-PAGE under non-reducing conditions.

P1291 | LIGHT Human Recombinant DataSheet

Biomolecule/Target: LIGHT

Synonyms: TNFSF14, HVEM-L, Tumor necrosis factor ligand superfamily member 14, Herpes virus entry mediator ligand, Herpesvirus entry mediator ligand, CD258, LIGHT

Alternates names: MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

Taglines: Apoptosis inducer

NCBI Gene ID #: 3562

NCBI Gene Symbol: IL3

Gene Source: Human

Accession #: P08700

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <0.1 EU/g

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.5 to 2 ng/mL.The activity was determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Results: 0.5 to 2 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17 to 45 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 20 mM Sodium Phosphate, pH 7.0.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A