LIMD1<sup>---<-sup> CRISPR Stable Knockout HeLa Cell Line | T6357

LIMD1^{---<-sup> CRISPR Stable Knockout HeLa Cell Line | T6357}

LIMD1 knockout was conducted via CRISPR to establish the LIMD1-/- CRISPR Stable Knockout HeLa Cell Line. LIMD1 is an adapter or scaffold protein and is required for silencing of microRNA (miRNA)-mediated genes. However, the function and mechanism for the gene is poorly understood. This cell line may be used to study the functions and roles of LIMD1.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

40,000 - 50,000 cells/cm²

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Co-immunoprecipitation

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C