LIN54 Antibody | 7933

LIN54 Antibody | 7933 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.
The immunogen is located within the last 50 amino acids of LIN54.

Research Area: Cell Cycle

Tested Application: E, WB, ICC, IF

Application: LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 88 kDa
Observed: 82 kDa

Validation: N/A

Isoform: N/A

Purification: LIN54 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: LIN54 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: LIN54 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: LIN-54, JC8.6, CXCDC1, MIP120

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1) . LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2) .