LIX (CXCL6), Murine Recombinant | P1293

LIX is a CXC chemokine that signals through the CXCR2 receptor. It is expressed in monocytes, platelets, endothelial cells, and mast cells. LIX is a chemoattractant for neutrophils. The two naturally occurring variants of LIX; LIX 1-78 (GCP-2) and LIX 9-78 (GCP-2), contain 78 and 70 amino acid residues, respectively. LIX contains the four conserved cysteine residues present in CXC chemokines, and also contains the ‘ELR’ motif common to CXC chemokine that bind to the CXCR1 and CXCR2 receptors. Recombinant murine LIX is a 9.8 kDa protein containing 92 amino acid residues.

P1293 | LIX CXCL6 Murine Recombinant DataSheet

Biomolecule/Target: LIX (CXCL6)

Synonyms: CXCL6, GCP-2 (human), Granulocyte Chemotactic Protein-2

Alternates names: IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.

Taglines: Chemoattractant for neutrophils

NCBI Gene ID #: 3569

NCBI Gene Symbol: IL6

Gene Source: Human

Accession #: P05231

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: > 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level:

Activity (Specifications/test method): TF-1 cells were stimulated with human recombinant IL-6 for 36 hrs and cell proliferation was measured using the Quick Cell Proliferation Colorimetric Assay Kit Plus (Cat. No. K302).

Biological activity: EC50 < 1 ng/mL

Results: EC50 <0.7 ng/mL

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus and has a calculated MW of 22.8 kDa (30-212aa). Under reducing conditions the protein migrates as ~23-29 kDa bands in SDS-PAGE.

Concentration: N/A

Appearance: Lyophilized

Physical form description: N/A

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water.

Amino acid sequence: N/A