LSD1 Antibody| F41978-0.4ML/R34467-100UG

LSD1 Antibody| F41978-0.4ML/R34467-100UG | Gentaur UK, US & Europe Distribution

Background: LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Format: Purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human, Mouse

Predicted Spacies Reactivity: N/A

Application: WB, IHC, IF, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:50-1:100, Immunofluorescence: 1:10-1:50

Application Note: Titration of the LSD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This LSD1 antibody is available for research use only.

Purity: Purified

Immunogen: A portion of amino acids 108-142 from the human protein was used as the immunogen for this LSD1 antibody.

Storage: Aliquot the LSD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A