Lyn B, Active | 7715

Lyn is a 56 kd tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck and the v-yes protein as well as to the gene products of v-fgr and v-src. Northern hybridization analysis showed that a 3.2-kilobase Lyn mRNA was expressed in a variety of tissues of the human fetus (1). Lyn is expressed preferentially in B cells and can be coimmunoprecipitated with IgM suggesting that Lyn is physically associated with membrane-bound IgM, and participates in antigen-mediated signal transduction (2). Crosslinking of membrane-bound IgM with antibody induces rapid increase in activities of Lyn and Lyn-associated phosphatidylinositol 3-kinase (3). Crosslinking of B-cell antigen receptor also induces association of Lyn with an 85-kDa noncatalytic subunit of phosphatidylinositol 3-kinase. Thus, Lyn is functionally associated with membrane-bound IgM and participates in B-cell antigen receptor-mediated signaling.

7715 | Lyn B Active DataSheet

Biomolecule/Target: N/A

Synonyms: Lyn B

Alternates names: Lyn B

Taglines: A key enzyme involved in the regulation of cell activation

NCBI Gene ID #: 4283

NCBI Gene Symbol: CXCL9

Gene Source: N/A

Accession #: Q07325

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 180 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: The ED range = 10 -100 ng/ml, determined by the dose dependent chemotaxis with human lymphocytes cultured in the presence of IL-2.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 85.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: Reconstitute in HO to a concentration of 1 mg/ml. The solution can be diluted into other buffered solutions or store at –20°C for future use.

Amino acid sequence: N/A