M-RIP polyclonal Antibody | CAS7516

M-RIP polyclonal Antibody | CAS7516 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IP IHC

Application Range: WB: 1:5000~1:10000 IP:1:2000~1:8000 IHC:1:50~1:100

Background: Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: M-RIP polyclonal Antibody detects endogenous levels of M-RIP protein.

Molecular Weight: ~ 130-160kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Myosin phosphatase Rho-interacting protein; M-RIP;Rho-interacting protein 3;RIP3;p116Rip;MPRIP

Immunogen: Recombinant protein of human M-RIP.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: