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MacConkey Agar (Dehydrated) | CM0007B

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SKU:
CM0007B-GEN
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Description

MacConkey Agar (Dehydrated) | CM0007B

Detect, isolate and enumerate coliforms and intestinal pathogens in water, dairy products and biological specimens with Thermo Scientific™ Oxoid™ MacConkey Agar (Dehydrated). This differential medium gives a number of diagnostic indications such as bile tolerance, colony morphology and chromogenesis. MacConkey Agar corresponds to the medium recommended by the World Health Organizaton1, the Department of Health2 for the bacterial examination of water.

Use MacConkey Agar for the cultivation of pathogens which may be present in a variety of specimens.

  • Easy-to-read: easy to differentiate Escherichia coli (red non-mucoid colonies) from Staphylococcus spp. (pale pink opaque colonies) and Pseudomonas aeruginosa (green-brown) colonies.
  • Recommended by World Health Organizaton1, the Department of Health2.

Typical Formula*
Peptone  20.0
Lactose   10.0
Bile salts  5.0
Sodium chloride  5.0
Neutral red  0.075
Agar  12.0
* adjusted as required to meet performance standards

Directions
Suspend 52g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at
121°C for 15 minutes. Cool to 50°C. Mix well and pour into sterile Petri dishes.
Physical Characteristics
Straw/pink, free-flowing powder
Colour on reconstitution - dark red
Moisture level - less than 7%
pH 7.4 ± 0.2 at 25°C
Clarity - clear
Gel strength - firm, comparable to 12.0g/litre of agar

Microbiological Tests Using Optimum Inoculum Dilution
Control Medium: Tryptone Soya Agar
Reactions after incubation at 37°C for 18-24 hours
Medium is challenged with 10-100 colony-forming units
Salmonella typhimurium ATCC® 14028 1-3mm straw colonies
Salmonella nottingham NCTC 7832 1-3mm straw colonies
Shigella flexneri ATCC® 12022 1-4mm straw colonies
Klebsiella pneumoniae ATCC® 29665 1-3mm red/pink, mucoid colonies
Pseudomonas aeruginosa ATCC® 27853 1-3mm straw colonies
Escherichia coli ATCC® 25922 1-2mm pink/red colonies
Proteus mirabilis ATCC® 29906 1-2mm straw colonies, no swarming
Enterococcus faecalis ATCC® 29212 0.5-1mm red colonies
Staphylococcus aureus ATCC® 25923 0.5-1mm pink colonies
A satisfactory result is represented by recovery of positive strains equal to or greater than 70% of the control
medium.

 

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