MAD1L1 Antibody (N-term) [APR03677G]

MAD1L1 Antibody (N-term) [APR03677G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This MAD1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-151 amino acids from the N-terminal region of human MAD1L1.

Cellular Localisation: Nucleus. Chromosome, centromere, kinetochore. Nucleus envelope Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Co- localizes with TPR at the nucleus envelope during interphase and throughout the cell cycle (PubMed:22351768, PubMed:18981471) . From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody (PubMed:9546394) . Localizes to kinetochores during prometaphase (PubMed:22351768, PubMed:29162720) . Does not localize to kinetochores during metaphase (PubMed:29162720) Colocalizes with NEK2 at the kinetochore (PubMed:14978040) . Colocalizes with IK at spindle poles during metaphase and anaphase (PubMed:22351768) .

Molecular Weight: 83067

Clone: Polyclonal

Gene Name: MAD1L1

Gene ID: 8379

Function: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate (PubMed:10049595, PubMed:20133940, PubMed:29162720) . Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling (PubMed:29162720) .

Summary: Tissue Location: [Isoform 1]: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, Mitotic checkpoint MAD1 protein homolog, HsMAD1, hMAD1, Tax-binding protein 181, MAD1L1, MAD1, TXBP181