MAD2L2 Antibody (C-term) [APR04652G]

MAD2L2 Antibody (C-term) [APR04652G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: M

Specificity: This MAD2L2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 198-231 amino acids from the C-terminal region of human MAD2L2.

Cellular Localisation: Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Chromosome. Note=Recruited to sites of chromosomal double-stranded breaks during G1 and S phase of the cell cycle

Molecular Weight: 24334

Clone: Polyclonal

Gene Name: MAD2L2

Gene ID: 10459

Function: Adapter protein able to interact with different proteins and involved in different biological processes (PubMed:11459825, PubMed:11459826, PubMed:17719540, PubMed:17296730, PubMed:19443654, PubMed:29656893) . Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis (PubMed:20164194) . Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions (PubMed:20164194) . Component of the shieldin complex, which plays an important role in repair of DNA double-stranded breaks (DSBs) (PubMed:29656893) . During G1 and S phase of the cell cycle, the complex functions downstream of TP53BP1 to promote non-homologous end joining (NHEJ) and suppress DNA end resection (PubMed:29656893) . Mediates various NHEJ-dependent processes including immunoglobulin class-switch recombination, and fusion of unprotected telomeres (PubMed:29656893) . May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1 (PubMed:17296730) . Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle (PubMed:11459825, PubMed:17719540) . Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation (PubMed:19443654) .

Summary: Tissue Location: Ubiquitously expressed.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, IHC-P, IF

Dilution: IHC-P--1:100~500 IF--1:25 WB--1:1000

Synonyms: Mitotic spindle assembly checkpoint protein MAD2B, Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, REV7 homolog, hREV7, MAD2L2, MAD2B, REV7