MAGEA3, human recombinant | 9245

Melanoma-associated antigen 3, also known MAGEA3, is a member of the MAGE gene family, which comprises 12 known genes, of which 6 are expressed in tumors. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGEA3 is a tumor-specific antigen widely expressed in solid and hematologic malignancies, but not in normal tissues, with the exception of testis and placenta. Therefore, MAGEA3 is an excellent candidate tumor antigen. Recombinant human MAGEA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

9245 | MAGEA3 human recombinant DataSheet

Biomolecule/Target: MAGEA3

Synonyms: CT1.3, HIP8, HYPD, MAGE3, MZ2 D

Alternates names: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Taglines: Member of the MAGE gene family that enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases.

NCBI Gene ID #: 3586

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P22301

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: 90%

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of IL-4 using ’s Quick Cell Proliferation colorimetric Assay Kit Plus (Cat. No. K302). The ED50 is typically in a single digit ~ 1ng/ml.

Biological activity:

Results:

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19 kDa with a 6×His tag at the C-terminus

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: Liquid

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Amino acid sequence: Ser 19 – Asn 178