MAGEL2 Antibody (C-term) [APR05707G]

MAGEL2 Antibody (C-term) [APR05707G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This MAGEL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-529 amino acids from the C-terminal region of human MAGEL2.

Cellular Localisation: Early endosome. Cytoplasm {ECO:0000250|UniProtKB:Q9QZ04}. Nucleus {ECO:0000250|UniProtKB:Q9QZ04}. Note=Recruited to retromer-containing endosomes via interaction with VPS35. Colocalizes with CLOCK and ARNTL/BMLA1 in the cytoplasm, and with PER2 in the cytoplasm and nucleus (By similarity) . {ECO:0000250|UniProtKB:Q9QZ04, ECO:0000269|PubMed:23452853}

Molecular Weight: 132822

Clone: Polyclonal

Gene Name: MAGEL2

Gene ID: 54551

Function: Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (PubMed:23452853) . Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (By similarity) .

Summary: Tissue Location: Expressed in placenta, fetal and adult brain. Not detected in heart and small intestine, very low levels in fibroblasts Not expressed in brain of a Prader-Willi patient

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:2000

Synonyms: MAGE-like protein 2, Necdin-like protein 1, Protein nM15, MAGEL2, NDNL1