Malachite Green ELISA Kit - E4941

E4941 | Malachite Green ELISA Kit DataSheet

Malachite Green is a triarylmethane dye that applications in numerous industries. It is used in the pigment industry wherein it is used as a dye to color silk, paper, wool, and leather. In the aquaculture industry, it is used as an ectoparasiticide, fungicide, and antiseptic. It is also widely used as a food coloring agent and food additive. However, the use of malachite green has been controversial due to its toxic effects. The dye is reported to cause mutagenesis, carcinogenesis, immunological, reproductive, and respiratory toxicity. ’s Malachite Green ELISA kit is used to quantitatively measure Malachite Green in muscle tissue samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Malachite Green antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Malachite Green in the samples.

Alternate Name : Aniline green, Basic green 4, Diamond green B, Victoria green B

Tag Line : A Competitive ELISA kit to quantitatively measure Malachite Green in tissue samples

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Tissue

Species Reactivity : Universal

Applications : Competitive ELISA kit to quantitatively measure Malachite Green in tissue samples

Features and Benefits : Cross reaction: Malachite Green 100%, Crystal violet 80%, Leucomalachite green (oxidized) 100%, Leucocrystal violet (oxidized) 80% This ELISA kit is used for in vitro quantitative determination of Malachite Green Sensitivity: 0.025ppb Detection Limit: 0.1ppb for tissue (muscle) samples