MAPRE1-MAPRE3 Doxycycline Inducible Double Knockout HeLa Cell Line | T6174

MAPRE1-MAPRE3 Doxycycline Inducible Double Knockout HeLa Cell Line | T6174

This human cell line stably expresses single guide RNA (sgRNA) targeting an early exon of MAPRE1 (microtubule-associated protein RP/EB family member 1) and of MAPRE3 (microtubule-associated protein RP/EB family member 3). Cas9 expression is induced by the presence of doxycycline, enabling inducible knockout of the genes of interest. A PiggyBac transposon system was used to introduce Cas9 while guide RNAs were introduced using lentiviral infection.

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Cervix

Growth Properties:

Adherent

Morphology:

Epithelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

24 - 34 hours

Seeding Density:

40,000 - 50,000 cells/cm²

Markers:

EB1/MAPRE1 and EB3/MAPRE3, doxycycline-inducible Cas9 nuclease

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 50% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot; 2) Immunofluorescence

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C