MART-1/Melan-A, human recombinant | 4823

MART-1 (also known as Melan-A) is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. SubCellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Human MART-1 is purified by proprietary chromatographic techniques.

4823 | MART-1/Melan-A human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Melanoma antigen recognized by T-cells 1, Protein Melan-A, Antigen SK29-AA, Antigen LB39-AA

Alternates names: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.

Taglines: A melanocyte lineage marker

NCBI Gene ID #: 3586

NCBI Gene Symbol: IL10

Gene Source: Human

Accession #: P22301

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): The specific activity was determined by the dose-dependent simulation of the proliferation of MC/9 cells (mouse mast cell line) in the presence of IL-4 using ’s Quick Cell Proliferation colorimetric Assay Kit Plus (Cat. No. K302). The ED50 is typically in a single digit ~ 1ng/ml.

Biological activity:

Results:

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19 kDa with a 6×His tag at the C-terminus

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized with no additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Amino acid sequence: Ser 19 – Asn 178