MART-1/Melan-A, mouse recombinant | 4824

MART-1 (also known as Melan-A) is a melanocyte differentiation antigen or marker recognized by autologous cytotoxic T lymphocytes. It is a membrane protein with one transmembrane peptide. Melan-A is expressed by a large proportion of melanoma tumors, both melanotic and amelanotic and by melanoma cell lines. It is present in melanocytes of normal skin and retina, nevi and in more than 85% of melanomas. Melan-A/MART-1 is not expressed by non-melanoma malignancies. It is recognized by autologous cytotoxic T lymphocytes. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Mouse MART-1 is purified by proprietary chromatographic techniques.

4824 | MART-1/Melan-A mouse recombinant DataSheet

Biomolecule/Target: MART-1/Melan-A

Synonyms: Melanoma antigen recognized by T-cells 1, Antigen LB39-AA, Antigen SK29-AA, MART-1, Melan-A

Alternates names: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

Taglines: Pure mouse recombinant full length MART-1/Melan-A.

NCBI Gene ID #: 3592

NCBI Gene Symbol: IL12A

Gene Source: Human

Accession #: P29459

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.1 to 0.5 ng/mL ng/mL. The specific activity was determined by the dose-dependent release of IFN-gamma from the human NK92 cell line in presence of 20 ng/mL rIL-2.

Results: 0.1 to 0.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 57 kDa, heterodimer, glycosylated

Concentration: N/A

Appearance: Liquid

Physical form description: MART-1/Melan-A protein is in 50 mM Tris, pH 7.5, 100 mM NaCl, 20 mM -mercaptoethanol, and 20%Glycerol.

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A