Description
MAX Antibody | 45-088 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen: The immunogen for this antibody is: C-EEPQSRKKLRMEAS
Research Area: Homeostasis
Tested Application: E, WB, IF, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3kDa according to NP_002373.3) . Recommended concentration: 0.01-0.03ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10ug/ml.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificiy: This antibody is expected to recognize both reported isoforms (NP_002373.3; NP_660087.1) .
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: MAX, MAX protein, MGC10775, MGC11225, MGC18164, MGC34679, MGC36767, myc-associated factor X, helix-loop-helix zipper protein, MYC associated factor X, bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1
User Note: N/A
BACKGROUND: N/A