MBP-Tag Mouse monoclonal Antibody | MB2012

MBP-Tag Mouse monoclonal Antibody | MB2012 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: All

Application: WB

Application Range: WB: 1:5000~1:10000

Background: MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site. Alternatively, the intact fusion protein can be specifically eluted from the resin by the addition of excess free maltose. Subsequent to elution, MBP fusion protein can be visualized either by western blot analysis or immunoprecipitation using antibodies specific for the MBP-tag. This Antibody recognizes MBP (Maltose binding protein) TAG in some expression systems.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: MBP-tag mouse monoclonal Antibody detects endogenous levels of internal, C-terminal, and N-terminal MBP -tagged recombinant proteins.

Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: MBP

Immunogen: Synthetic peptide MBP-Tag.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: Affinity-chromatography

Pathway: