MCM7 Antibody [MCM7/1466] | 33-725

MCM7 Antibody [MCM7/1466] | 33-725 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Amino acids 195-319 were used as the immunogen for the MCM7 antibody.

Research Area: Other

Tested Application: Flow, IF, IHC-P, WB

Application: IF: 0.5-1 ug/ml
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
WB: 0.5-1 ug/ml
IHC (FFPE) : 0.5-1 ug/ml for 30 min at RT
The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: MCM7/1466

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]