MCP-1/MCAF Antibody | XP-5219

MCP-1/MCAF Antibody | XP-5219 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Rat

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat MCP-1/MCAF (Macrophage/Monocyte chemotactic protein-1) .

Research Area: Chemokines & Cytokines, Antibody Pairs, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat MCP-1/MCAF (100 ng/mL) , a concentration of 2.0 - 4.0 μg/mL of this antibody is required.
ELISA:
To detect Rat MCP-1/MCAF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat MCP-1/MCAF.
Sandwich:
To detect Rat MCP-1 (MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat MCP-1 (MCAF) (XP-5219Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat MCP-1 (MCAF) .
Western Blot:
To detect Rat MCP-1/MCAF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1/MCAF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-Rat MCP-1/MCAF specific antibody was purified by affinity chromatography employing immobilized Rat MCP-1/MCAF matrix.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: MCP-1/MCAF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: MCP-1, Scya2, Sigje, Je, Mcp1, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, MCP-1

User Note: Centrifuge vial prior to opening.

BACKGROUND: N/A