MD-2 Antibody [1A2E3] | PM-4847

MD-2 Antibody [1A2E3] | PM-4847 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.

Research Area: Innate Immunity

Tested Application: E, WB, IHC-P, IF, ICC

Application: MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in recombinnat protein; Immunohistochemistry in human samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 95-108 - MD-2 Recombinant Protein

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: MD-2 Monoclonal Antibody is Protein A purified.

Clonality: Monoclonal

Clone: 1A2E3

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MD-2 monoclonal antibody can be stored at -20˚C, stable for one year.

Alternate Name: MD-2 Antibody [1A2E3] : MD2, MD-2, ly-96, ESOP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: MD-2 Monoclonal Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) . TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS) , requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.