MD-2 Antibody | 3289

MD-2 Antibody | 3289 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-MD-2 antibody (3289) was raised against a peptide corresponding to 13 amino acids near the center of human MD-2.
The immunogen is located within the last 50 amino acids of MD-2.

Research Area: Innate Immunity

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-1 μg/mL; IHC: 2-2.5 μg/mL; IF: 10-20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 2: Cat. No. 1466 - Rat Spleen Tissue Lysate

Positive Control 3: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 19kD
Observed: 26 kD (Post-modification: 2 N-linked glycosylations)

Validation:

Recombinant Protein Test (Figure 1) : Anti-MD-2 antibodies (3289) detected human MD-2 recombinant protein at different concentrations.

Isoform: Human MD-2 has 2 isoforms, including isoform 1 (160aa, 18kD) and isoform 2 (130aa, 15kD) . Mouse MD-2 has one isoform (160aa, 18kD) and Rat MD-2 also has one isoform (160aa, 18kD) . 3289 can detect human, mouse and rat.

Purification: MD-2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: MD-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: MD-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: MD-2 Antibody: MD2, MD-2, ly-96, ESOP-1, ESOP1, MD2, Lymphocyte antigen 96, Ly-96

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: MD-2 Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) . TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS) , requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.