Description
MD-2 Antibody [9F1B1] | PM-4845 | Gentaur UK, US & Europe Distribution
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Mouse monoclonal MD-2 antibody was raised against a recombinant protein corresponding to amino acids 2 to 160 of human MD-2.
Research Area: Innate Immunity
Tested Application: E, WB, IHC-P, IF
Application: MD-2 antibody can be used for detection of MD-2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 95-108 - MD-2 Recombinant Protein
Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: MD-2 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Clonality: Monoclonal
Clone: 9F1B1
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: MD-2 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: MD-2 monoclonal antibody can be stored at -20˚C, stable for one year.
Alternate Name: MD-2 Antibody [9F1B1] : MD2, MD-2, ly-96, ESOP-1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: MD-2 Monoclonal Antibody: MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) . TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS) , requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.