MDH1 protein (His tag) | 80R-1427

MDH1 protein (His tag) | 80R-1427 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Conjugated Proteins

Research Area: Cancer

Short Description: Purified recombinant Human MDH1 protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 1-334 amino acids: MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH

Tag/conjugate: His tag

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 95% pure

Methode of Purification: N/A

Source: N/A

Concentration: 1 mg/ml

Form & Buffer: Supplied as a liquid in 20mM Tris-HCl Buffer, pH 8.0, containing 10% glycerol.

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: Specific activity is > 8 units/mg, and is defined as the amount of enzyme that cleaves 1uM of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 and 25 DEG C.

Usage Recommendations: N/A

Assay Information: 1. Prepare assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid. 2. Add recombinant MDH protein with various concentrations (0.1ug, 0.5ug, 1ug) in assay buffer. 3. Mix by inversion, equilibrate to 1 DEg C, and monitor at A340nm until the value is constant using a spectrophotometer. 4. Record the increase at A340nm for approximately 2 minutes.