ME1 Antibody | 7721

ME1 Antibody | 7721 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1.
The immunogen is located within the first 50 amino acids of ME1.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P

Application: ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.

Positive Control 1: Cat. No. 1210 - HEK293 Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 63 kDa
Observed: 63 kDa

Validation: N/A

Isoform: N/A

Purification: ME1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ME1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ME1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ME1 Antibody: MES, HUMNDME, NADP-dependent malic enzyme, Malic enzyme 1, NADP-ME

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1) . ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2) . It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3) . The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3, 4) . ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5) .