ME2 Antibody | 7723

ME2 Antibody | 7723 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2.
The immunogen is located within amino acids 340 - 390 of ME2.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P, IF

Application: ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.

Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 64 kDa
Observed: 60 kDa

Validation: N/A

Isoform: N/A

Purification: ME2 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ME2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ME2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: ME2 Antibody: ODS1, NAD-dependent malic enzyme, mitochondrial, Malic enzyme 2, NAD-ME

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1) . It is related to malic enzyme 1 (ME1) , a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2) . The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3) . Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4) .