MERS-CoV Spike RBD Protein | P1625

MERS-CoV is a coronavirus which causes the Middles East Respiratory Syndrome (MERS). MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein is responsible for allowing the virus to attach and fuse with the membrane of a host cell. Spike glycoprotein is cleaved into three chains: S1, S2’ and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays an important role in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

P1625 | MERS-CoV Spike RBD Protein DataSheet

Biomolecule/Target :

Synonyms: Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; Middle East respiratory syndrome-related coronavirus; RBD spike protein; S1 protein; RBD; EMC/2012; E2; Peplomer protein; receptor binding domain

Alternates names: Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; Middle East respiratory syndrome-related coronavirus; RBD spike protein; S1 protein; RBD; EMC/2012; E2; Peplomer protein; receptor binding domain

Taglines: S1 glycoprotein containing a receptor binding domain

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Coronavirus

Accession #: K0BRG7, AFS88936

Recombinant: TRUE

Source: Baculovirus

Purity by SDS-PAGE: ≥ 90% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level: < 1 EU/ug of the protein as determined by LAL method

Activity (Specifications/test method):

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 28.2 kDa

Concentration: 0.5 mg/ml (determined by Absorbance at 280nm)

Appearance: Liquid

Physical form description:

Reconstitution Instructions:

Amino acid sequence: The target protein is expressed with the sequence (aa 358-606) of MERS-CoV Spike RBD fused to His-tag at the C-terminus.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans