Metronidazole ELISA Kit - E4948

E4948 | Metronidazole ELISA Kit DataSheet

Metronidazole is an antibacterial and antiprotozoal agent that belongs to the class of nitroimidazoles. It is used to treat parasitic infections such as trichomoniasis, giardiasis, gastrointestinal infections, and bacterial infections in humans. Although the exact mechanism is unknown, it is believed that an intermediate formed by the reduction of metronidazole by anaerobic organisms binds to the DNA and electron-transport proteins of these organisms and inhibits nucleic acid synthesis. The antibiotic has been demonstrated to cause cancer in mice and rats when exposed to high concentrations for a long period. Similar risks may be associated with humans. Due to its carcinogenic properties, this drug is prohibited from extra-label use in food animals. ’s Metronidazole ELISA kit is used to quantitatively measure Metronidazole in muscle tissue, honey, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Metronidazole antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Metronidazole in the samples.

Alternate Name : Metronidazole

Tag Line : A Competitive ELISA kit for quantitative measurement of Metronidazole in tissue, urine, and serum samples

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Tissue, Urine, Serum

Species Reactivity : Universal

Applications : Competitive ELISA kit for quantitative determination of Metronidazole in tissue, urine and serum samples

Features and Benefits : Detection Limit: 1.5ppb for muscle tissue, 1.5ppb for honey, 3ppb for egg Sensitivity: 1.5ppb This ELISA kit is used for in vitro quantitative determination of Metronidazole Cross reaction: Metronidazole (MNZ) 100%, Dimetridazole (DMZ) 68%