METTL4 Antibody (C-term) [APR30595G]

METTL4 Antibody (C-term) [APR30595G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, Rat

Specificity: This METTL4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-344 amino acids from the C-terminal region of human METTL4.

Cellular Localisation: Nucleus. Cytoplasm, cytosol. Mitochondrion matrix

Molecular Weight: 54041

Clone: Polyclonal

Gene Name: METTL4 {ECO:0000303|PubMed:31913360, ECO:0000312|HGNC:HGNC:24726}

Gene ID: 64863

Function: N (6) -adenine-specific methyltransferase that can methylate both RNAs and DNA (PubMed:31913360, PubMed:32183942) . Acts as a N (6) - adenine-specific RNA methyltransferase by catalyzing formation of N6, 2'-O-dimethyladenosine (m6A (m) ) on internal positions of U2 small nuclear RNA (snRNA) : methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation (PubMed:31913360) . Internal m6A (m) methylation of snRNAs regulates RNA splicing (PubMed:31913360) . Also able to act as a N (6) -adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N (6) - methyladenosine) (PubMed:32183942) . The existence of N (6) - methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N (6) - adenine-specific DNA methyltransferase activity of METTL4 in vivo (PubMed:32203414) . Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N (6) -methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending (PubMed:32183942) . N (6) -methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (By similarity) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, FC, IHC-P-Leica

Dilution: IHC-P-Leica--1:500 FC--1:25 WB--1:2000

Synonyms: Methyltransferase-like protein 4, 211-, METTL4