Midkine, human recombinant | 4479

Human Midkine (MK) is a new member of the heparin-binding neurotrophic factor family. MK and PTN (Pleiotrophin) are structural homologs, and are highly conserved among species. MK plays important roles in development and carcinogenesis and has several important biological effects, including promotion of neurite extension and neuronal survival. Recombinant midkine is a 13.4 kDa protein, comprising of 123 amino acid residues.

4479 | Midkine human recombinant DataSheet

Biomolecule/Target: Midkine

Synonyms: NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.

Alternates names: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Taglines: A basic heparin-binding growth factor

NCBI Gene ID #: 7124

NCBI Gene Symbol: TNF

Gene Source: Human

Accession #: P01375

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: > 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): N/A

Biological activity: ED is typically < 0.9 ng/mL. The specific activity was determined by the dose-dependent cytotoxity of the TNF alpha sensitive cell line L-929 in the presence of Actinomycin D.

Results: < 0.9 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17 kDa, non-disulfide bonded homotrimer, glycosylated

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from 0.4X PBS, pH7.4

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.

Amino acid sequence: N/A