MIF Antibody | 45-888

MIF Antibody | 45-888 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Rat, Mouse

Immunogen: The immunogen for this antibody is: C-NAANVGWNNSTFA

Research Area: Cancer, Infectious Disease

Tested Application: E, WB, IHC, EIA

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 13kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5kDa according to NP_002406.1) . Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MIF captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml

Specificiy: N/A

Positive Control 1: Cat. No. 1314 - Human Thymus Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: MIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor) , HGNC:7097, GIF, GLIF, MMIF, glycosylation-inhibiting factor, phenylpyruvate tautomerase, macrophage migration inhibitory factor

User Note: N/A

BACKGROUND: N/A