MIF/Macrophage migration inhibitory factor | E21-H33

MIF/Macrophage migration inhibitory factor | E21-H33 | Gentaur UK, US & Europe Distribution

Short Description: Recombinant Human Macrophage migration inhibitory factor is produced by our E.coli expression system and the target gene encoding Met1-Ala115 is expressed.

Shipping: Dry Ice/ice packs

Formulation: Supplied as a 0.2 m filtered solution of 20mM PB, 150mM NaCl, 20% Glycerol, pH7.4.

Storage: Store at < -20C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Reconstitution: N/A

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/g (1 IEU/g) as determined by LAL test.

Background: Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without asignal sequence .Secretion occurs nonclassically via an ABCA1 transporter.Pro-inflammatory cytokine.Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites ofinflammation suggests a role as mediator in regulating the function of acrophages in host defense.Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase anddopachrome tautomerase activity (in vitro) , but the physiological substrate is not known. It is not clearwhether the tautomerase activity has any physiological relevance, and whether it is important for cytokineactivity.

Species: Human

Expression System: E.coli