MIP-1a Antibody [1.2_3E8-2H6-2B6] | XP-5734-M

MIP-1a Antibody [1.2_3E8-2H6-2B6] | XP-5734-M | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: The monoclonal antibody was produced in C3H/HeJ mice using recombinant hMIP-1α as the immunizing antigen.

Research Area: Immunology, Chemokines & Cytokines, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1α (100 ng/mL) , a concentration of 5.0-10.0 μg/mL of this antibody is required.
ELISA:
In a sandwich ELISA (assuming 100 μL/well) , a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 2000 pg/mL of recombinant human MIP-1α when used with our biotinylated antigen affinity purified anti-human MIP-1α (XP-5232Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.
Western Blot:
To detect hMIP-1α by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-1α is 1.0-2.0 ng/lane, under non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG1 antibody was purified from ascites fluid by SAS precipitation followed by ion exchange column.

Clonality: Monoclonal

Clone: 1.2_3E8-2H6-2B6

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: MIP-1α lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Alternate Name: Macrophage inflammatory protein 1 alpha, CCL 3, MIP 1 alpha, MIP1a, SCYA 3, Heparin binding chemotaxis protein, G0S19-1, MIP1A, SCYA3, C-C motif chemokine 3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha,

User Note: Centrifuge vial prior to opening.

BACKGROUND: Macrophage Inflammatory Protein 1 alpha and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation.