MIP-2 Antibody | XP-5149

MIP-2 Antibody | XP-5149 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Rat

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Rat GROβ/MIP-2 (Rat GROβ/Macrophage Inflammatory Protein-2) .

Research Area: Chemokines & Cytokines, Signal Transduction, Homeostasis, Antibody Pairs, Functional Assays

Tested Application: E, WB, Neut

Application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat GROβ/MIP-2 (100 ng/mL) , a concentration of 3.0 - 5.0 μg/mL of this antibody is required.
ELISA:
To detect Rat GROβ/MIP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant Rat GROβ/MIP-2.
Sandwich: To detect Rat GROβ/MIP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat GROβ/MIP-2 (XP-5149Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat GROβ/MIP-2.
Western Blot:
To detect Rat GROβ/MIP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat GROβ/MIP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-Rat GROβ/MIP-2 specific antibody was purified by affinity chromatography employing immobilized Rat GROβ/MIP-2 matrix.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: GROB/MIP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: Mip-2, Scyb2, Cinc3, Mip-2, Mip2, C-X-C motif chemokine 2, Cytokine-induced neutrophil chemoattractant 3, CINC-3

User Note: Centrifuge vial prior to opening.

BACKGROUND: Macrophage inflammatory protein 2 (MIP-2) is a chemokine that attracts neutrophils, and its secretion from intestinal epithelial cells is enhanced by inflammatory stimuli such as interleukin 1beta.