miR-34a Stably Expressing K562 Cell Line | T6450

miR-34a Stably Expressing K562 Cell Line | T6450

K-562 cells were purchased from ATCC (ATCC CCL-243™) and transfected with miR34a lentivirus vectors. Stable clones were established with puromycin (0.6µM) selection. miR34a has been used in sickle cell anemia research by studying its relation to fetal hemoglobin expression (Ward et al.). K-562 cells can be use for in vitro assay of natural killer cells (Pross, et al.). Pross HF, et al. Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells. IX. The quantitation of natural killer cell activity. J. Clin. Immunol. 1: 51-63, 1981. PubMed: 7334070

Biosafety:

II

Organism:

Human (H. sapiens)

Source Organ:

Bone Marrow

Growth Properties:

Suspension

Morphology:

Lymphoblast

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

miR-34a overexpression GFP Puromycin resistant Vector information: shMIMIC Human Lentiviral micro RNA has-miR-34a-5p (Cat#VSH6185-202526453) Company : Dharmacon (ThermoFisher)

Applications:

For Research Use Only

Doner Gender:

Female

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow V medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Flow cytometry; 2) RT-qPCR; 3) Western blot; 2) Densitometry quantification

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C